Production of radiolabeled e peptide

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Ea4 peptide of rainbow trout pro-IGF-I has been shown to exert anti-cancer activities in human cancer cell lines. Kou and Chen (2003) have shown that the trout Ea-4 peptide could bind specifically with a binding component on the membrane of neuroblastoma cells (SK-NF-1). In order to characterize this binding component, a radio-labeled ligand with high specific activity will be required. In this study, effort was made to label trout Ea4-peptide by phosphorylation by protein kinase A (PKA) with radioactive ATP. Recombinant PKA4 peptide was prepared by adding 5 amino acid residues to the carboxyl-terminal of the trout Ea4-peptide. Following partial purification, recombinant PKA4 peptide and Ea4-peptide were active in mitogenesis in mouse fibroblast cells and morphological differentiation in SK-N-SH neuroblastoma cells. By protein kinase reaction, recombinant PKA4 peptide was labeled with 32P-ATP. Both rtEa4 and PKA4 peptides were labeled with 32P using the PKA enzyme. PKA4 peptide was labeled at levels over 10 times the expected values. Four serine phosphorylation sites and 3 threonine phosphorylation sites on the rtEa4 peptide may have contributed to the phosphorylation.

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Oorspronkelijke releasedatum: 20 juni 2010

Aantal pagina's: 76

Hoofdauteur: Christine Sallum

Hoofduitgeverij: Lap Lambert Academic Publishing

Product breedte: 152 mm

Product hoogte: 5 mm

Product lengte: 229 mm

Verpakking breedte: 152 mm

Verpakking hoogte: 5 mm

Verpakking lengte: 229 mm

Verpakkingsgewicht: 122 g

EAN: 9783838374901

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